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Chemical imaging of lignocellulosic biomass by CARS microscopy

C. Pohling ; Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; A. Duarte ; T. Buckup ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; M. Motzkus
Journal of Biophotonics (1864-063X). Vol. 7 (2014), 1-2, p. 126-134.
[Artikel, refereegranskad vetenskaplig]

Chemical and structural composition of wood biomass is studied by label-free and chemically specific Coherent Anti-Stokes Raman Scattering (CARS) microscopy. A concept developed for assignment and semi-quantitative imaging of sample components; cellulose, hemicellulose, and lignin; by multiplex CARS microspectroscopy and subsequent data analysis is presented. Specific imaging without fluorescence backround is achieved an order of magnitude faster compared with conventional Raman microscopy. Laser polarization control yield information on molecular arrangement in wood fibers. Narrowband CARS excitation of single vibrations allows for three-dimensional volume imaging. Thus, CARS microscopy has potential as an important instrument for characterization of lignocellulosic materials.

Nyckelord: CARS microscopy, biomass, wood, cellulose, lignin, xylan

Denna post skapades 2014-07-04.
CPL Pubid: 200201


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)


Biofysikalisk kemi

Chalmers infrastruktur