CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Determination of exosome concentration in solution using surface plasmon resonance spectroscopy.

Deborah LM Rupert (Institutionen för teknisk fysik, Biologisk fysik) ; Cecilia Lässer ; Maria Eldh ; Stephan Block (Institutionen för teknisk fysik, Biologisk fysik) ; Vladimir P. Zhdanov (Institutionen för teknisk fysik, Biologisk fysik) ; Jan Lötvall ; Marta Bally (Institutionen för teknisk fysik, Biologisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Analytical Chemistry (0003-2700). Vol. 86 (2014), 12, p. 5929–5936.
[Artikel, refereegranskad vetenskaplig]

Exosomes are cell-secreted nanometer-sized extracellular vesicles that have been reported to play an important role in intercellular communication. They are also considered potential diagnostic markers for various health disorders, and intense investigations are presently directed towards their use as carriers in drug-delivery and gene-therapy applications. This has generated a growing need for sensitive methods capable of accurately and specifically determining the concentration of exosomes in complex biological fluids. Here, we explore the use of label-free surface-based sensing with surface plasmon resonance (SPR) read-out to determine the concentration of exosomes in solution. Human mast cell secreted exosomes carrying the tetraspanin membrane protein CD63 were analyzed by measuring their diffusion-limited binding rate to an SPR sensor surface functionalized with anti-CD63 antibodies. The concentration of suspended exosomes was determined by first converting the SPR response into surface-bound mass. The increase in mass uptake over time was then related to the exosome concentration in solution using a formalism describing diffusion-limited binding under controlled flow conditions. The proposed quantification method is based on a calibration and control measurements performed with proteins and synthetic lipid vesicles and takes into account i) the influence of the broad size distribution of the exosomes on the surface coverage, ii) the fact that their size is comparable to the ~150 nm probing depth of SPR, and iii) possible deformation of exosomes upon adsorption. Under those considerations, the accuracy of the concentration determination was estimated to be better than ±50% and significantly better if exosome deformation is negligible.

Denna post skapades 2014-05-27. Senast ändrad 2017-10-03.
CPL Pubid: 198546


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)
Krefting Research Centre (GU)


Biologisk fysik

Chalmers infrastruktur