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CARS microscopy of Alzheimer's diseased brain tissue

Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Juris Kiskis (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Helen Fink (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Lena Nyberg (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; J. Thyr ; J. Li
MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES XIV: Progress in Biomedical Optics and Imaging - Proceedings of SPIE (1605-7422). Vol. 8948 (2014),
[Konferensbidrag, refereegranskat]

Alzheimera's disease (AD) is a progressive neurodegenerative disorder currently without cure, characterized by the presence of extracellular plaques surrounded by dystrophic neurites. In an effort to understand the underlying mechanisms, biochemical analysis (protein immunoblot) of plaque extracts reveals that they consist of amyloid-beta (Aβ) peptides assembled as oligomers, protofibrils and aggregates. Their spatial distribution has been confirmed by Thioflavin-S or immuno-staining with fluorescence microscopy. However, it is increasingly understood that the protein aggregation is only one of several mechanism that causes neuronal dysfunction and death. This raises the need for a more complete biochemical analysis. In this study, we have complemented 2-photon fluorescence microscopy of Thioflavin-S and Aβ immuno-stained human AD plaques with CARS microscopy. We show that the chemical build-up of AD plaques is more complex and that Aβ staining does not provide the complete picture of the spatial distribution or the molecular composition of AD plaques. CARS images provide important complementary information to that obtained by fluorescence microscopy, motivating a broader introduction of CARS microscopy in the AD research field.

Denna post skapades 2014-05-08. Senast ändrad 2015-06-12.
CPL Pubid: 197715


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)


Medicinsk bioteknologi

Chalmers infrastruktur