CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Biofilm formation on nanostructured hydroxyapatite-coated titanium

Emma Westas (Institutionen för kemi- och bioteknik, Teknisk ytkemi) ; M. Gillstedt ; J. Lönn-Stensrud ; E.M. Bruzell ; Martin Andersson (Institutionen för kemi- och bioteknik, Teknisk ytkemi)
Journal of Biomedical Materials Research - Part A (1549-3296). Vol. 102 (2014), 4, p. 1063-1070.
[Artikel, refereegranskad vetenskaplig]

Biofilm formation on medical devices is a common cause of implant failure, especially regarding implants that breach the epithelial tissue, so-called transcutaneous implants. Nanotechnology and the development of new nanomaterials have given the opportunity to design nanotextured implant surfaces. Such surfaces have been studied using various in vitro methods showing that nanosized features strongly benefit bone cell growth. However, little is known on how nanostructured features affect biofilm formation. The aim of this study was therefore to examine the shape- and chemical-dependent effect of a nanostructured hydroxyapatite (HA) coating on the degree of Staphylococcus epidermidis biofilm formation. Three different types of nanosized HA particles having different shapes and calcium to phosphate ratios were compared to uncoated turned titanium using safranin stain in a biofilm assay and confocal laser scanning microscopy (CLSM) for assessment of biofilm biomass and bacterial volume, respectively. No difference in biofilm biomass was detected for the various surfaces after 6 h incubation with S. epidermidis. Additionally, image analysis of CLSM Z-stacks confirmed the biofilm assay and showed similar results. In conclusion, the difference in nanomorphology and chemical composition of the surface coatings did not influence the adhesion and biofilm formation of S. epidermidis.

Nyckelord: biofilm , hydroxyapatite , implant , nanomorphology , Staphylococcus epidermidis

Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2014-04-11. Senast ändrad 2016-11-08.
CPL Pubid: 196589


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Teknisk ytkemi (2005-2014)


Hållbar utveckling
Innovation och entreprenörskap (nyttiggörande)
Biologiska vetenskaper
Medicinska material och protesteknik

Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:

Protein and Cell Interactions with Nanostructured Surfaces