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In situ forming spruce xylan-based hydrogel for cell immobilization

Volodymyr Kuzmenko (Institutionen för mikroteknologi och nanovetenskap, Bionanosystem) ; Daniel Hägg (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Guillermo Toriz (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi)
Carbohydrate Polymers (0144-8617). Vol. 102 (2014), p. 862-868.
[Artikel, refereegranskad vetenskaplig]

An in situ forming spruce xylan-based hydrogel was synthesized in two steps with the intended use of cell encapsulation and in vivo delivery. First, bioconjugate was obtained through the reaction of glucuronic acid groups from xylan backbone with tyramine (TA). After that, the gelation process was enabled by enzymatic crosslinking of the phenol-containing TA-xylan conjugate. Exhibiting an exponential increase in the storage modulus, a 3D gel network was formed in about 20s. The designed gel showed extensive swelling and retained its mechanical integrity for more than two months. Mesenchymal stem cells were encapsulated in the hydrogel and cultured for one week. The cells retained their adipogenic differentiation capacity inside the gel, as verified by lipid accumulation. From these facts, we conclude that spruce xylan is a promising precursor for in situ forming hydrogels and should be evaluated further for tissue engineering purposes.



Denna post skapades 2014-04-08.
CPL Pubid: 196425

 

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Institutioner (Chalmers)

Institutionen för mikroteknologi och nanovetenskap, Bionanosystem (2007-2015)
Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)

Ämnesområden

Polymerkemi

Chalmers infrastruktur