CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Enzymes Immobilized in Mesoporous Silica: a Physical-Chemical Perspective

Nils Carlsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Hanna Gustafsson (Institutionen för kemi- och bioteknik, Teknisk ytkemi ; SuMo Biomaterials) ; Christian Thörn (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Lisbeth Olsson (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Björn Åkerman (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Krister Holmberg (Institutionen för kemi- och bioteknik, Teknisk ytkemi)
Advances in Colloid and Interface Science (0001-8686). Vol. 205 (2014), p. 339-360.
[Artikel, refereegranskad vetenskaplig]

Mesoporous materials as support for immobilized enzymes have been explored extensively during the last two decades, primarily not only for biocatalysis applications, but also for biosensing, biofuels and enzyme-controlled drug delivery. The activity of the immobilized enzymes inside the pores is often different compared to that of the free enzymes, and an important challenge is to understand how the immobilization affects the enzymes in order to design immobilization conditions that lead to optimal enzyme activity. This review summarizes methods that can be used to understand how material properties can be linked to changes in enzyme activity. Real-time monitoring of the immobilization process and techniques that demonstrate that the enzymes are located inside the pores is discussed by contrasting them to the common practice of indirectly measuring the depletion of the protein concentration or enzyme activity in the surrounding bulk phase. We propose that pore filling (pore volume fraction occupied by proteins) is the best standard for comparing the amount of immobilized enzymes at themolecular level, and present equations to calculate pore filling from the more commonly reported immobilized mass. Methods to detect changes in enzyme structure upon immobilization and to study the microenvironment inside the pores are discussed in detail. Combining the knowledge generated from these methodologies should aid in rationally designing biocatalyst based on enzymes immobilized in mesoporous materials.



Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2014-04-06. Senast ändrad 2016-04-22.
CPL Pubid: 196308

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)
Institutionen för kemi- och bioteknik, Teknisk ytkemi (2005-2014)
SuMo Biomaterials
Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Materialvetenskap
Kemi
Fysikalisk kemi
Yt- och kolloidkemi
Biokatalys och enzymteknik

Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:


Immobilization of feruloyl esterases in mesoporous silica