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Monitoring the osmotic response of single yeast cells through force measurement in the environmental scanning electron microscope

Anna Jansson (Institutionen för teknisk fysik, Eva Olsson Group ; SuMo Biomaterials) ; Alexandra Nafari (Institutionen för mikroteknologi och nanovetenskap) ; Kristina Hedfalk ; Eva Olsson (Institutionen för teknisk fysik, Eva Olsson Group ) ; K. Svensson ; Anke Sanz-Velasco (Institutionen för mikroteknologi och nanovetenskap, Bionanosystem)
Measurement science and technology (0957-0233). Vol. 25 (2014), 2, p. Art. no. 025901.
[Artikel, refereegranskad vetenskaplig]

We present a measurement system that combines an environmental scanning electron microscope (ESEM) and an atomic force microscope (AFM). This combination enables studies of static and dynamic mechanical properties of hydrated specimens, such as individual living cells. The integrated AFM sensor provides direct and continuous force measurement based on piezoresistive force transduction, allowing the recording of events in the millisecond range. The in situ ESEM-AFM setup was used to study Pichia pastoris wild-type yeast cells. For the first time, a quantified measure of the osmotic response of an individual yeast cell inside an ESEM is presented. With this technique, cell size changes due to humidity variations can be monitored with nanometre accuracy. In addition, mechanical properties were extracted from load-displacement curves. A Young's modulus of 13-15 MPa was obtained for the P. pastoris yeast cells. The developed method is highly interesting as a complementary tool for the screening of drugs directed towards cellular water transport activity and provides new possibilities of studying mechanosensitive regulation of aquaporins.

Nyckelord: atomic force microscopy, cell viability, environmental scanning electron microscopy, in situ, osmotic response, single cell characterization, yeast cell



Denna post skapades 2014-03-14. Senast ändrad 2016-07-11.
CPL Pubid: 195001

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Eva Olsson Group (2012-2015)
SuMo Biomaterials
Institutionen för mikroteknologi och nanovetenskap
Institutionen för kemi och molekylärbiologi (GU)
Institutionen för mikroteknologi och nanovetenskap, Bionanosystem (2007-2015)

Ämnesområden

Fysik

Chalmers infrastruktur

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