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Real-time monitoring of surface-confined platelet activation on TiO2

Angelika Kunze (Institutionen för teknisk fysik, Biologisk fysik) ; Camilla Hesse ; Sofia Svedhem (Institutionen för teknisk fysik, Biologisk fysik)
Colloids and Surfaces B: Biointerfaces (0927-7765). Vol. 116 (2014), p. 446-451.
[Artikel, refereegranskad vetenskaplig]

For the development of advanced hemocompatible biomaterial functions, there is an unmet demand for in vitro evaluation techniques addressing platelet-surface interactions. We show that the quartz crystal microbalance with dissipation (QCM-D) monitoring technique, here combined with light microscopy, provides a surface sensitive technique that allows for real-time monitoring of the activation and aggregation of the surface-confined platelets on TiO2. The QCM-D signal monitored during adhesion and activation of platelets on TiO2 coated surfaces was found to be different in platelet-poor and platelet-rich environment although light microscopy images taken for each of the two cases looked essentially the same. Interestingly, aggregation of activated platelets was only observed in a protein-rich environment. Our results show that a layer of plasma proteins between the TiO2 surface and the platelets strongly influences the coupling between the platelets and the underlying substrate, explaining both the observed QCM-D signals and the ability of the platelets to aggregate. © 2014 The Authors.

Nyckelord: Aggregation, Platelet activation, QCM-D, TiO2

Denna post skapades 2014-03-13. Senast ändrad 2015-03-26.
CPL Pubid: 194875


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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)


Biologisk fysik

Chalmers infrastruktur