CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

On-chip light sheet illumination enables diagnostic size and concentration measurements of membrane vesicles in biofluids

H. Deschout ; K. Raemdonck ; S. Stremersch ; P. Maoddi ; G. Mernier ; P. Renaud ; S. Jiguet ; A. Hendrix ; M. Bracke ; R. Van den Broecke ; Magnus Röding (Institutionen för matematiska vetenskaper, matematisk statistik ; SuMo Biomaterials) ; Mats Rudemo (Institutionen för matematiska vetenskaper, matematisk statistik) ; J. Demeester ; S. C. De Smedt ; F. Strubbe ; K. Neyts ; K. Braeckmans
Nanoscale (2040-3364). Vol. 6 (2014), 3, p. 1741-1747.
[Artikel, refereegranskad vetenskaplig]

Cell-derived membrane vesicles that are released in biofluids, like blood or saliva, are emerging as potential non-invasive biomarkers for diseases, such as cancer. Techniques capable of measuring the size and concentration of membrane vesicles directly in biofluids are urgently needed. Fluorescence single particle tracking microscopy has the potential of doing exactly that by labelling the membrane vesicles with a fluorescent label and analysing their Brownian motion in the biofluid. However, an unbound dye in the biofluid can cause high background intensity that strongly biases the fluorescence single particle tracking size and concentration measurements. While such background intensity can be avoided with light sheet illumination, current set-ups require specialty sample holders that are not compatible with high-throughput diagnostics. Here, a microfluidic chip with integrated light sheet illumination is reported, and accurate fluorescence single particle tracking size and concentration measurements of membrane vesicles in cell culture medium and in interstitial fluid collected from primary human breast tumours are demonstrated.



Denna post skapades 2014-02-27. Senast ändrad 2016-04-04.
CPL Pubid: 194215

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
SuMo Biomaterials

Ämnesområden

Kemi

Chalmers infrastruktur