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Two modes of exocytosis in an artificial cell

Lisa J. Mellander ; Michael E. Kurczy (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Neda Najafinobar (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Johan Dunevall (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Andrew G Ewing (Institutionen för kemi- och bioteknik, Analytisk kemi ; Institutionen för kemi och molekylärbiologi) ; Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Analytisk kemi)
Scientific Reports (2045-2322). Vol. 4 (2014), p. (article nr.) 3847.
[Artikel, refereegranskad vetenskaplig]

The details of exocytosis, the vital cell process of neuronal communication, are still under debate with two generally accepted scenarios. The first mode of release involves secretory vesicles distending into the cell membrane to release the complete vesicle contents. The second involves partial release of the vesicle content through an intermittent fusion pore, or an opened or partially distended fusion pore. Here we show that both full and partial release can be mimicked with a single large-scale cell model for exocytosis composed of material from blebbing cell plasma membrane. The apparent switching mechanism for determining the mode of release is demonstrated to be related to membrane tension that can be differentially induced during artificial exocytosis. These results suggest that the partial distension mode might correspond to an extended kiss-and-run mechanism of release from secretory cells, which has been proposed as a major pathway of exocytosis in neurons and neuroendocrine cells.

Nyckelord: KISS-AND-RUN, PHEOCHROMOCYTOMA PC12 CELLS, CHROMAFFIN CELLS, ELECTROCHEMICAL CYTOMETRY, SECRETORY VESICLES, LIPID NANOTUBES, QUANTAL SIZE, FUSION PORE, SPIKE FEET, RELEASE



Denna post skapades 2014-02-20. Senast ändrad 2015-02-26.
CPL Pubid: 194000

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)

Ämnesområden

Kemi

Chalmers infrastruktur

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