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Continuous Cultivation of Dilute-Acid Hydrolysates to Ethanol by Immobilized Saccharomyces cerevisiae

Mohammad J. Taherzadeh (Institutionen för kemisk reaktionsteknik) ; Claes Niklasson (Institutionen för kemisk reaktionsteknik) ; Ria Millati (Institutionen för kemisk reaktionsteknik)
Applied Biochemistry and Biotechnology (0273-2289). Vol. 95 (2001), 1, p. 45-57.
[Artikel, refereegranskad vetenskaplig]

The continuous cultivation of immobilized Saccharomyces cerevisiae CBS 8066 on dilute-acid hydrolysates of forest residuals was investigated. The yeast cells were immobilized in 2–4% Ca-alginate beads. The 2% beads were not stable. However, the 3 and 4% beads were stable for at least 3 wk when an extra resource of calcium ions was available in the medium. The continuous cultivation of a dilute-acid hydrolysate by the immobilized cells at dilution rates of 0.3, 0.5, and 0.6 h−1 resulted in 86, 83, and 79% sugar consumption, respectively, and an ethanol yield between 0.45 and 0.48 g/g. The hydrolysate was fermentable at a dilution rate of 0.1 h−1 in a free-cell system but washed out at a dilution rate of 0.2 h−1. The continuous cultivation of a more inhibiting hydrolysate was not successful by either free- or immobilized-cell systems even at a low dilution rate of 0.07 h−1. However, when the hydrolysate was overlimed, it was fermentable by the immobilized cells at a dilution rate of 0.2 h−1.

Nyckelord: Saccharomyces cerevisiae ; dilute–acid hydrolysate; ethanol; glucose; immobilization.

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Denna post skapades 2014-02-03. Senast ändrad 2014-02-03.
CPL Pubid: 193358


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Institutionen för kemisk reaktionsteknik (1972-2001)



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