CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Artificial nanotube connections and transport of molecular cargo between mammalian cells

Haijiang Zhang (Institutionen för kemi- och bioteknik) ; Shijun Xu (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Gavin Jeffries (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Aldo Jesorka (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Nano Communication Networks (1878-7789). Vol. 4 (2013), 4, p. 197-204.
[Artikel, refereegranskad vetenskaplig]

Lipid nanotubes interconnecting mammalian cells, sometimes referred to as tunneling nanotubes, have recently received increased attention, as their importance in intercellular transport and communication has been recognized. We present a set of microfabrication procedures for the deliberate formation of intercellular nanotube links between single mammalian cells, employing microneedle manipulation protocols, developed earlier for nanotube-vesicle networks. Cell-to-cell connections were established, and their effectiveness for intercellular transport of molecular cargo was demonstrated. We initiate transport of Ca2 + ions and fluorescein diphosphate, a prefluorescent enzyme substrate, between cells, and demonstrate the validity and effectiveness of artificially created nanotube connections for cell-to-cell communication. We thus provide a new experimental model for probing cell-to-cell communication, which we deem the foundation for man-made network architectures involving biological cells. This model can greatly facilitate fundamental studies of cell-to-cell communication modes, the exchange of cell constituents and components, and the dynamics of biochemical reactions in native network environments. © 2013 Elsevier Ltd. All rights reserved.

Nyckelord: Intercellular communication , Intercellular nanotube network , Lipid nanotube , Tunneling nanotube



Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2013-11-18. Senast ändrad 2016-06-30.
CPL Pubid: 186994

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kemi- och bioteknik (2005-2014)
Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Nanovetenskap och nanoteknik
Biofysikalisk kemi

Chalmers infrastruktur