CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Transcriptome signatures in Helicobacter pylori-infected mucosa identifies acidic mammalian chitinase loss as a corpus atrophy marker

Intawat Nookaew (Institutionen för kemi- och bioteknik, Systembiologi) ; Kaisa Thorell (Institutionen för kemi- och bioteknik, Systembiologi ; Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi) ; Kuntal Worah (Institutionen för kemi- och bioteknik ; Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi) ; Shugui Wang ; Martin Lloyd Hibberd ; Henrik Sjövall ; Sven Pettersson ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi) ; Samuel B Lundin
BMC Medical Genomics (1755-8794). Vol. 6 (2013), 41,
[Artikel, refereegranskad vetenskaplig]

The majority of gastric cancer cases are believed to be caused by chronic infection with the bacterium Helicobacter pylori, and atrophic corpus gastritis is a predisposing condition to gastric cancer development. We aimed to increase understanding of the molecular details of atrophy by performing a global transcriptome analysis of stomach tissue. Biopsies from patients with different stages of H. pylori infection were taken from both the antrum and corpus mucosa and analyzed on microarrays. The stages included patients without current H. pylori infection, H. pylori-infected without corpus atrophy and patients with current or past H. pylori-infection with corpus-predominant atrophic gastritis. Using clustering and integrated analysis, we found firm evidence for antralization of the corpus mucosa of atrophy patients. This antralization harbored gain of gastrin expression, as well as loss of expression of corpus-related genes, such as genes associated with acid production, energy metabolism and blood clotting. The analyses provided detailed molecular evidence for simultaneous intestinal metaplasia (IM) and spasmolytic polypeptide expressing metaplasia (SPEM) in atrophic corpus tissue. Finally, acidic mammalian chitinase, a chitin-degrading enzyme produced by chief cells, was shown to be strongly down-regulated in corpus atrophy. Transcriptome analysis revealed several gene groups which are related to development of corpus atrophy, some of which were increased also in H. pylori-infected non-atrophic patients. Furthermore, loss of acidic chitinase expression is a promising marker for corpus atrophy.

Nyckelord: Corpus gastritis, Gastric cancer, Integrated analysis, Acidic mammalian chitinase



Denna post skapades 2013-11-05. Senast ändrad 2015-11-26.
CPL Pubid: 186081

 

Läs direkt!

Lokal fulltext (fritt tillgänglig)

Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)
Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi (GU)
Institutionen för kemi- och bioteknik (2005-2014)
Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition (GU)

Ämnesområden

Klinisk medicin

Chalmers infrastruktur

C3SE/SNIC (Chalmers Centre for Computational Science and Engineering)