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Self-calibrated concentration measurements of polydisperse nanoparticles

Magnus Röding (Institutionen för matematiska vetenskaper, matematisk statistik ; SuMo Biomaterials) ; H. Deschout ; K. Braeckmans ; Aila Särkkä (Institutionen för matematiska vetenskaper, matematisk statistik) ; Mats Rudemo (Institutionen för matematiska vetenskaper, matematisk statistik)
Journal of Microscopy (0022-2720). Vol. 252 (2013), 1, p. 79-88.
[Artikel, refereegranskad vetenskaplig]

Summary Quantitative characterization of nanoparticles, e.g. accurate estimation of concentration distributions, is critical to many pharmaceutical and biological applications. We present a method that enables for the first time highly accurate size and absolute concentration measurements of polydisperse nanoparticles in solution, based on fluorescence single particle tracking, that are self-calibrated in the sense that the detection region volume is estimated based on the tracking data. The method is evaluated using simulations and experimental data of polystyrene nanospheres in water/sucrose solution. In addition, the method is used to quantify aggregation and clearance of different types of liposomes after intravenous injection in rats, where additional and more accurate information can be obtained that was previously unavailable, which can help elucidate their usefulness as drug carriers.

Nyckelord: nanoparticles, size distribution, liposomal drug carriers, polydispersity, fluorescence single particle tracking, Concentration, single-particle tracking, biological-fluids, quantum dots, fluorescence, cells



Denna post skapades 2013-10-28. Senast ändrad 2016-07-07.
CPL Pubid: 185757

 

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Institutioner (Chalmers)

Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
SuMo Biomaterials

Ämnesområden

Nanoteknik

Chalmers infrastruktur