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The photoinduced transformation of fluorescent DNA base analogue tC triggers DNA melting

S. Preus ; S. Jonck ; M. Pittelkow ; Anke Dierckx (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Thitinun Karpkird (Institutionen för kemi- och bioteknik) ; Bo Albinsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Marcus Wilhelmsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Photochemical & Photobiological Sciences (1474-905X). Vol. 12 (2013), 8, p. 1416-1422.
[Artikel, refereegranskad vetenskaplig]

While fluorescent analogues of the canonical nucleobases have proven to be highly valuable in a large number of applications, up until today, fluorescent DNA base analogues remain virtually inapplicable for single-molecule fluorescence experiments which require extremely bright and photostable dyes. Insight into the photodegradation processes of these fluorophores is thus a key step in the continuous development towards dyes with improved performances. Here, we show that the commercially available fluorescent nucleobase analogue tC under intense long-term illumination and in the presence of O-2 is degraded to form a single photoreaction product which we suggest to be the sulfoxide form of tC. The photoproduct is characterized by a blue-shifted absorption and a less intense fluorescence compared to that of tC. Interestingly, when tC is positioned inside double-stranded DNA this photodriven conversion of tC to its photoproduct greatly reduces the duplex stability of the overall double helix in which the probe is positioned. Since tC can be excited selectively at 400 nm, well outside the absorption band of the natural DNA bases, this observation points towards the application of tC as a general light-triggered switch of DNA duplex stability.



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Denna post skapades 2013-09-17. Senast ändrad 2015-08-25.
CPL Pubid: 183449

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)
Institutionen för kemi- och bioteknik (2005-2014)

Ämnesområden

Livsvetenskaper
Nanovetenskap och nanoteknik
Fysikalisk kemi
Biofysikalisk kemi
Spektroskopi

Chalmers infrastruktur

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Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions