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In situ laccase treatment enhances the fermentability of steam-exploded wheat straw in SSCF processes at high dry matter consistencies

Antonio D. Moreno (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Elia Tomas-Pejo (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; D. Ibarra ; M. Ballesteros ; Lisbeth Olsson (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
Bioresource Technology (0960-8524). Vol. 143 (2013), p. 337-343.
[Artikel, refereegranskad vetenskaplig]

This work evaluates the in situ detoxification of inhibitory lignocellulosic broths by laccases to facilitate their fermentation by the xylose-consuming Saccharomyces cerevisiae F12. Treatment of wheat straw slurries with laccases prior to SSCF processes decreased the total phenolic content by 50-80%, reducing the lag phase and increasing the cell viability. After laccase treatment, a negative impact on enzymatic hydrolysis was observed. This effect, together with the low enzymatic hydrolysis yields when increasing consistency, resulted in a decrease in final ethanol yields. Furthermore, when using high substrate loading (20% DM (w/v)), high concentration of inhibitors prevailed in broths and the absence of an extra nitrogen source led to a total cell growth inhibition within the first 24 h in non-treated samples. This inhibition of growth at 20% DM (w/v) was overcome by laccase treatment with no addition of nitrogen, allowing S. cerevisiae F12 to produce more than 22 g/L of ethanol.

Nyckelord: Lignocellulose, Bioethanol, Simultaneous saccharification and co-fermentation, In situ laccase, S. cerevisiae F12

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Denna post skapades 2013-09-16. Senast ändrad 2016-09-14.
CPL Pubid: 183429


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)


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Industriell bioteknik

Chalmers infrastruktur