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Improving biobutanol production in engineered Saccharomyces cerevisiae by manipulation of acetyl-CoA metabolism

Anastasia Krivoruchko (Institutionen för kemi- och bioteknik, Systembiologi) ; Cristina Serrano-Amatriain (Institutionen för kemi- och bioteknik, Systembiologi) ; Yun Chen (Institutionen för kemi- och bioteknik, Systembiologi) ; Verena Siewers (Institutionen för kemi- och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Journal of Industrial Microbiology & Biotechnology (1367-5435). Vol. 40 (2013), 9, p. 1051-1056.
[Artikel, refereegranskad vetenskaplig]

Recently, butanols (1-butanol, 2-butanol and iso-butanol) have generated attention as alternative gasoline additives. Butanols have several properties favorable in comparison to ethanol, and strong interest therefore exists in the reconstruction of the 1-butanol pathway in commonly used industrial microorganisms. In the present study, the biosynthetic pathway for 1-butanol production was reconstructed in the yeast Saccharomyces cerevisiae. In addition to introducing heterologous enzymes for butanol production, we engineered yeast to have increased flux toward cytosolic acetyl-CoA, the precursor metabolite for 1-butanol biosynthesis. This was done through introduction of a plasmid-containing genes for alcohol dehydrogenase (ADH2), acetaldehyde dehydrogenase (ALD6), acetyl-CoA synthetase (ACS), and acetyl-CoA acetyltransferase (ERG10), as well as the use of strains containing deletions in the malate synthase (MLS1) or citrate synthase (CIT2) genes. Our results show a trend to increased butanol production in strains engineered for increased cytosolic acetyl-CoA levels, with the best-producing strains having maximal butanol titers of 16.3 mg/l. This represents a 6.5-fold improvement in butanol titers compared to previous values reported for yeast and demonstrates the importance of an improved cytosolic acetyl-CoA supply for heterologous butanol production by this organism.

Nyckelord: Biobutanol, Biofuel, Acetyl-coenzyme A, Saccharomyces cerevisiae, Metabolic engineering, Synthetic biology

Denna post skapades 2013-09-10. Senast ändrad 2015-12-17.
CPL Pubid: 182976


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)


Bioinformatik och systembiologi

Chalmers infrastruktur