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MicroRNA regulate immune pathways in T-cells in multiple sclerosis (MS)

Margareta Jernås ; Clas Malmeström ; Markus Axelsson ; Intawat Nookaew (Institutionen för kemi- och bioteknik, Systembiologi) ; Hans Wadenvik ; Jan Lycke ; Bob Olsson
BMC Immunology (1471-2172). Vol. 14 (2013), 32,
[Artikel, refereegranskad vetenskaplig]

Background: MicroRNA are small noncoding RNA molecules that are involved in the control of gene expression. To investigate the role of microRNA in multiple sclerosis (MS), we performed genome-wide expression analyses of mRNA and microRNA in T-cells from MS patients and controls. Methods: Heparin-anticoagulated peripheral blood was collected from MS-patients and healthy controls followed by isolation of T-cells. MicroRNA and RNA from T-cells was prepared and hybridized to Affymetrix miR 2.0 array and Affymetrix U133Plus 2.0 Human Genome array (Santa Clara, CA), respectively. Verifications were performed with real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Results: We identified 2,452 differentially expressed genes and 21 differentially expressed microRNA between MS patients and controls. By Kolmogorov-Smirnov test, 20 of 21 differentially expressed microRNA were shown to affect the expression of their target genes, many of which were involved in the immune system. Tumor necrosis factor ligand superfamily member 14 (TNFSF14) was a microRNA target gene significantly decreased in MS. The differential expression of mir-494, mir-197 and the predicted microRNA target gene TNFSF14 was verified by real-time PCR and ELISA. Conclusion: These findings indicate that microRNA may be important regulatory molecules in T-cells in MS.

Nyckelord: Autoimmunity, T-cell; Microarray, MicroRNA

Denna post skapades 2013-08-19. Senast ändrad 2013-09-02.
CPL Pubid: 181798


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Institutioner (Chalmers)

Institutionen för medicin, avdelningen för molekylär och klinisk medicin (GU)
Institutionen för neurovetenskap och fysiologi (GU)
Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)
Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition (GU)
Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi (GU)


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