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Chitosan conjugates for DNA delivery

D. Paiva ; G. Ivanova ; M. D. Pereira ; Sofia Sousa Da Rocha (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
Physical Chemistry Chemical Physics (1463-9076). Vol. 15 (2013), 28, p. 11893-11899.
[Artikel, refereegranskad vetenskaplig]

Graft copolymers of chitosan and maltodextrin were synthesized by reductive amination of a low molecular weight chitosan. The degree of substitution is 70% or above, as quantified by nuclear magnetic resonance spectroscopy, at molar ratios of chitosan glucosamine units and maltodextrin of 1 : 1 and 1 : 5. The high substitution degree generates a water-soluble compound at pH 7.4 by reducing the amino groups of chitosan. In addition, the copolymer self-assembles into spherical nanoparticles with diameters of about 300 nm. The mass polymer/DNA ratios at the isoelectric point are within the range of 3-3.5 for chitosan-maltodextrin nanoparticles as determined by electrophoretic mobility measurements. These results confirm that DNA interacts efficiently with the copolymer nanoparticles indicating a potential application of the system for DNA delivery.



Denna post skapades 2013-08-13.
CPL Pubid: 181238

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Kemiteknik

Chalmers infrastruktur