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Nonlinear nearfield microscopy

Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Juris Kiskis (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Henning Hagman (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Multiphoton Microscopy in the Biomedical Sciences Xiii (0277-786X). Vol. 8588 (2013),
[Konferensbidrag, refereegranskat]

Higher-order nonlinearity of light-matter interactions, such as second and third harmonic generation (SHG & THG) and Coherent anti-Stokes Raman Scattering (CARS) can be used for improving spatial resolution in microscopy as a consequence of the confinement of the nonlinear polarization to the high-intensity region of the focal volume. However, the resolution is limited to similar to 300 nm, not sufficient to resolve macromolecules or nanostructures of interest in the bio-, life- and nano-sciences. In the strive to push the resolution beyond the diffraction limit, allowing for nanoscale imaging, we have equipped a nonlinear optical microscope with a scanning-probe setup operated in tapping-mode feedback. A tapered, gold-coated, open-aperture tip with an aperture diameter of similar to 150 nm is scanned over the sample, probing the nonlinear nearfield generated by free-beam excitation. First nonlinear coherent Raman nearfield images of biological macromolecules and metallic nanostructures are shown. Limitations and future challenges with nonlinear nearfield microscopy are discussed.

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Denna post skapades 2013-08-12. Senast ändrad 2015-06-12.
CPL Pubid: 181089


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)


Nanovetenskap och nanoteknik
Optisk fysik

Chalmers infrastruktur