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A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study

R. G. E. van Eijsden ; C. Stassen ; L. Daenen ; S. E. Van Mulders ; P. M. Bapat ; Verena Siewers (Institutionen för kemi- och bioteknik, Systembiologi) ; K. V. Y. Goossens ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi) ; F. R. Delvaux ; P. Van Hummelen ; B. Devreese ; R. G. Willaert
Biotechnology Letters (0141-5492). Vol. 35 (2013), 6, p. 891-900.
[Artikel, refereegranskad vetenskaplig]

Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored directly after sampling. It has already been described that RNAlater can be used to store tissues and cells in a way that the RNA quality and quantity are preserved. In this paper, we demonstrate that quaternary ammonium salts (RNAlater) are also suitable to preserve and store samples from Saccharomyces cerevisiae for later use with the four major omics-technologies. Moreover, it is shown that RNAlater also preserves the cell morphology and the potential to recover growth, permitting microscopic analysis and yeast cell culturing at a later stage.

Nyckelord: Fluxomics; Genomics; Omics; RNAlater; Saccharomyces cerevisiae; Transcriptomics



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Denna post skapades 2013-07-04. Senast ändrad 2014-10-27.
CPL Pubid: 179815

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Livsvetenskaper
Bioinformatik och systembiologi

Chalmers infrastruktur