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Single Cell Amperometry Reveals Glycocalyx Hinders the Release of Neurotransmitters During Exocytosis

Raphaël Trouillon ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi- och bioteknik, Analytisk kemi)
Analytical Chemistry (0003-2700). Vol. 85 (2013), 9, p. 4822-4828.
[Artikel, refereegranskad vetenskaplig]

The diffusional hindrance of the glycocalyx along the cell surface on exocytotic peaks, observed with single cell amperometry, was investigated. Partial digestion of the glycocalyx with neuraminidase led to the observation of faster peaks, as shown by varied peak parameters. This result indicates that diffusion of small molecules in the partially digested glycocalyx is 2.2 faster than in the intact glycocalyx. Similarly, neutralization of the negative charges present in the cell microenvironment led to faster peak kinetics. The analysis of the vesicular efflux indicates that the diffusion coefficient of dopamine at the cell surface is at most 45% of the diffusion coefficient in free solution. This study shows that the glycocalyx plays an important role in the diffusion kinetics of processes along the cell surface, including exocytotic events.

Nyckelord: adrenal chromaffin cells, to-substrate contacts, extracellular-matrix, fusion pore, sialic-acid, pc12 cells, electrophoretic mobility, endothelial-cells, chemical-analysis, vesicular volume



Denna post skapades 2013-06-27. Senast ändrad 2013-07-05.
CPL Pubid: 179410

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)

Ämnesområden

Analytisk kemi

Chalmers infrastruktur