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The real catecholamine content of secretory vesicles in the CNS revealed by electrochemical cytometry

D. M. Omiatek ; A. J. Bressler ; Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Analytisk kemi) ; A. M. Andrews ; M. L. Heien ; Andrew G Ewing (Institutionen för kemi- och bioteknik, Analytisk kemi ; Institutionen för kemi och molekylärbiologi)
Scientific Reports (2045-2322). Vol. 3 (2013), p. artikel nr 1447.
[Artikel, refereegranskad vetenskaplig]

Resolution of synaptic vesicle neurotransmitter content has mostly been limited to the study of stimulated release in cultured cell systems, and it has been controversial as to whether synaptic vesicle transmitter levels are saturated in vivo. We use electrochemical cytometry to count dopamine molecules in individual synaptic vesicles in populations directly sampled from brain tissue. Vesicles from the striatum yield an average of 33,000 dopamine molecules per vesicle, an amount considerably greater than typically measured during quantal release at cultured neurons. Vesicular content was markedly increased by L-DOPA or decreased by reserpine in a time-dependent manner in response to in vivo administration of drugs known to alter dopamine release. We investigated the effects of the psychostimulant amphetamine on vesicle content, finding that vesicular transmitter is rapidly depleted by 50% following in vivo administration, supporting the "weak base hypothesis'' that amphetamine reduces synaptic vesicle transmitter and quantal size.

Nyckelord: substantia-nigra neurons, ventral tegmental area, quantal size, dopamine, release, capillary-electrophoresis, pc12 cells, rat-brain, exocytosis, amphetamine, midbrain



Denna post skapades 2013-04-05. Senast ändrad 2015-02-26.
CPL Pubid: 175367

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)
Institutionen för kemi och molekylärbiologi (GU)

Ämnesområden

Kemi

Chalmers infrastruktur