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Acoustic detection of melanosome transport in Xenopus laevis melanophores

Rickard Frost (Institutionen för teknisk fysik, Biologisk fysik) ; Elisabeth Norström ; Lovisa Bodin ; Christoph Langhammer (Institutionen för teknisk fysik, Kemisk fysik) ; Joachim Sturve ; Margareta Wallin ; Sofia Svedhem (Institutionen för teknisk fysik, Biologisk fysik)
Analytical Biochemistry (0003-2697). Vol. 435 (2013), 1, p. 10-18.
[Artikel, refereegranskad vetenskaplig]

Organelle transport studies are often performed using melanophores from lower vertebrates due to the ease of inducing movements of pigment granules (melanosomes) and visualizing them by optical microscopy. Here, we present a novel methodology to monitor melanosome translocation (which is a light-sensitive process) in the dark using the quartz crystal microbalance with dissipation monitoring (QCM-D) technique. This acoustic sensing method was used to study dispersion and aggregation of melanosomes in Xenopus laevis melanophores. Reversible sensor responses, correlated to optical reflectance measurements, were obtained by alternating addition and removal of melatonin (leading to melanosome aggregation) and melanocyte-stimulating hormone (MSH) (leading to melanosome dispersion). By confocal microscopy, it was shown that a vertical redistribution of melanosomes occurred during the dispersion/aggregation processes. Furthermore, the transport process was studied in the presence of cytoskeleton-perturbing agents disrupting either actin filaments (latrunculin) or microtubules (nocodazole). Taken together, these experiments suggest that the acoustic responses mainly originate from melanosome transport along actin filaments (located close to the cell membrane), as expected based on the penetration depth of the QCM-D technique. The results clearly indicate the potential of QCM-D for studies of intracellular transport processes in melanophores.

Nyckelord: Xenopus laevis; Melanophores; Melanosomes; QCM-D; Latrunculin; Nocodazole



Denna post skapades 2013-03-28. Senast ändrad 2015-02-11.
CPL Pubid: 175155

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)
Institutionen för biologi och miljövetenskap (GU)
Institutionen för teknisk fysik, Kemisk fysik (1900-2015)

Ämnesområden

Kemi

Chalmers infrastruktur

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