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Single-Cell Lipidomics: Characterizing and Imaging Lipids on the Surface of Individual Aplysia californica Neurons with Cluster Secondary Ion Mass Spectrometry

M. K. Passarelli ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi- och bioteknik, Analytisk kemi) ; N. Winograd
Analytical Chemistry (0003-2700). Vol. 85 (2013), 4, p. 2231-2238.
[Artikel, refereegranskad vetenskaplig]

Neurons isolated from Aplysia californica, an organism with a well-defined neural network, were imaged with secondary ion mass spectrometry, C-60-SIMS. A major lipid component of the neuronal membrane was identified as 1-hexadecyl-2-octadecenoyl-sn-glycero-3-phosphocholine [PC(16:0e/18:1)] using tandem mass spectrometry (MS/MS). The assignment was made directly off the sample surface using a C-60-QSTAR instrument, a prototype instrument that combines an ion source with a commercial electrospray ionization/matrix-assisted laser desorption ionization (ESI/MALDI) mass spectrometer, Normal phase liquid chromatography mass spectrometry (NP-LC-MS) was used to confirm the assignment. Cholesterol and vitamin E were also identified with in situ tandem MS analyses that were compared to reference spectra obtained from purified compounds. In order to improve sensitivity on the single-cell level, the tandem MS spectrum of vitamin E reference material was used to extract and compile all the vitamin E related peaks from the cell image. The mass spectrometry images heterogeneous distributions cif intact lipid species, PC(16:0e/18:1), vitamin E, and cholesterol on the surface of a single neuron. The ability to detect these molecules and determine their relative distribution on the single-cell level shows that the C-60-QSTAR is a potential platform for studying important biochemical processes, such as neuron degeneration.



Denna post skapades 2013-03-21. Senast ändrad 2013-03-26.
CPL Pubid: 174888

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)

Ämnesområden

Kemi

Chalmers infrastruktur