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Reconstruction and Evaluation of the Synthetic Bacterial MEP Pathway in Saccharomyces cerevisiae

Siavash Partow (Institutionen för kemi- och bioteknik, Systembiologi) ; Verena Siewers (Institutionen för kemi- och bioteknik, Systembiologi) ; L. Daviet ; M. Schalk ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
PLoS ONE (1932-6203). Vol. 7 (2012), 12, p. Article Number: e52498 .
[Artikel, refereegranskad vetenskaplig]

Isoprenoids, which are a large group of natural and chemical compounds with a variety of applications as e.g. fragrances, pharmaceuticals and potential biofuels, are produced via two different metabolic pathways, the mevalonate (MVA) pathway and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Here, we attempted to replace the endogenous MVA pathway in Saccharomyces cerevisiae by a synthetic bacterial MEP pathway integrated into the genome to benefit from its superior properties in terms of energy consumption and productivity at defined growth conditions. It was shown that the growth of a MVA pathway deficient S. cerevisiae strain could not be restored by the heterologous MEP pathway even when accompanied by the co-expression of genes erpA, hISCA1 and CpIscA involved in the Fe-S trafficking routes leading to maturation of IspG and IspH and E. coli genes fldA and fpr encoding flavodoxin and flavodoxin reductase believed to be responsible for electron transfer to IspG and IspH.

Nyckelord: deoxyxylulose phosphate-pathway, isoprenoid biosynthetic-pathway, escherichia-coli, isph protein, arabidopsis-thaliana, 4fe-4s protein, gene synthesis, transfer-rnas, sterol levels, in-vivo



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Denna post skapades 2013-02-28. Senast ändrad 2014-10-27.
CPL Pubid: 174248

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Livsvetenskaper
Kemiteknik

Chalmers infrastruktur