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In vitro and in vivo response to nanotopographically-modified surfaces of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and polycaprolactone.

Gianluca Giavaresi ; Matilde Tschon ; John H Daly ; John J Liggat ; Duncan S Sutherland ; Hossein Agheli (Institutionen för teknisk fysik, Kemisk fysik) ; Milena Fini ; Paola Torricelli ; Roberto Giardino
Journal of biomaterials science. Polymer edition (0920-5063). Vol. 17 (2006), 12, p. 1405-23.
[Artikel, refereegranskad vetenskaplig]

Colloidal lithography and embossing master are new techniques of producing nanotopography, which have been recently applied to improve tissue response to biomaterials by modifying the surface topography on a nano-scale dimension. A natural polyester (Biopol), 8% 3-hydroxyvalerate-component (D400G) and a conventional biodegradable polycaprolactone (PCL) were studied, both nanostructured and native forms, in vitro and in vivo. Nanopits (100-nm deep, 120-nm diameter) on the D400G surface were produced by the embossing master technique (Nano-D400G), while nanocylinders (160-nm height, 100-nm diameter) on the PCL surface were made by the colloidal lithography technique (Nano-PCL). L929 fibroblasts were seeded on polyesters, and cell proliferation, cytotoxic effect, synthetic and cytokine production were assessed after 72 h and 7 days. Then, under general anesthesia, 3 Sprague-Dawley rats received dorsal subcutaneous implants of nanostructured and native polyesters. At 1, 4 and 12 weeks the animals were pharmacologically euthanized and implants with surrounding tissue studied histologically and histomorphometrically. In vitro results showed significant differences between D400G and PCL in Interleukin-6 production at 72 h. At 7 days, significant (P < 0.05) differences were found in Interleukin-1beta and tumor necrosis factor-alpha release for Nano-PCL when compared to Nano-D400G, and for PCL in comparison with D400G. In vivo results indicated that Nano-D400G implants produced a greater extent of inflammatory tissue than Nano-PCL at 4 weeks. The highest vascular densities were observed for Nano-PCL at 4 and 12 weeks. Chemical and topographical factors seem to be responsible for the different behaviour, and from the obtained results a prevalence of chemistry on in vitro data and nanotopography on soft tissue response in vivo are hypothesized, although more detailed investigations are necessary in this field.

Nyckelord: Animals, Biocompatible Materials, chemistry, Blood Vessels, Cell Proliferation, Cell Survival, Cytokines, biosynthesis, Fibroblasts, cytology, Implants, Experimental, standards, Inflammation, Mice, Neovascularization, Physiologic, Polyesters, chemistry, Rats, Rats, Sprague-Dawley, Surface Properties, Time Factors, Tissue Engineering, methods

Denna post skapades 2013-02-25.
CPL Pubid: 174104


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Institutionen för teknisk fysik, Kemisk fysik (1900-2015)


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