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The Multifunctional Pipette. A Microfluidic Technology for the Biosciences

Alar Ainla (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Göteborg : Chalmers University of Technology, 2013. ISBN: 978-91-7385-818-2.- 204 s.
[Doktorsavhandling]

The theme of the work described in this thesis is the generation and application of liquid microenvironments in chemistry and bioscience using microfluidic devices. First, a computer controlled multi-stage dilution system to generate time-dependent chemical waves was developed, and its application was demonstrated on model biomembranes. Thereafter the focus was shifted towards spatial control of chemistry. Using a hydrodynamic flow confinement concept in an open liquid volume, we created a device coined “Multifunctional Pipette”. It features localized liquid handling at the single-cell size scale together with fast solution exchange. The technology has been refined and optimized to provide a feature-rich tool for biologists working with cells and tissues in microscopy experiments. Application examples include cell zeiosis, single-cell dose-response determination and ion-channel stimulation. Subsequent studies cover modifications and applications of this device, such as on-chip electrodes and electroporation, as well as uses in cell cultures, on tissue slices, and as an optofluidic thermometer. Finally, localized liquid handling has been applied to assemble 2-dimensional fluidic networks consisting of directly written supported lipid bilayers. This "Lab on a Membrane" toolbox allows rapid prototyping of 2D-fluidic circuits, to modify their chemistry and connectivity on-demand and to apply them in studies of molecular interactions.

Nyckelord: Microfluidics, microfabrication, PDMS, microfluidic dilution, microfluidic mixer, hydrodynamic flow confinement, microfluidic superfusion, single-cell analysis, supported lipid membranes, microfluidic temperature sensing.



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Denna post skapades 2013-02-13. Senast ändrad 2016-04-28.
CPL Pubid: 173557

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Nanovetenskap och nanoteknik
Analytisk kemi
Biofysikalisk kemi
Nanoteknik

Chalmers infrastruktur

NFL/Myfab (Nanofabrication Laboratory)

Relaterade publikationer

Inkluderade delarbeten:


A Microfluidic Diluter Based on Pulse Width Flow Modulation


A Microfluidic Pipette for Single-Cell Pharmacology


A multifunctional pipette


Single-cell electroporation using a multifunctional pipette


Examination

Datum: 2013-03-04
Tid: 13:15
Lokal: sal KA, Kemigården 4, Chalmers, Göteborg
Opponent: Prof. George M. Whitesides

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