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Automated segmentation of free-lying cell nuclei in Pap smears for malignancy-associated change analysis

Ramin Moshavegh (Institutionen för signaler och system) ; Babak Ehteshami Bejnordi (Institutionen för signaler och system) ; Andrew Mehnert (Institutionen för signaler och system, Digitala bildsystem och bildanalys) ; K. Sujathan ; P. Malm ; E. Bengtsson
34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012, San Diego, CA, 28 August - 1 September 2012 (1557-170X). p. 5372-5375. (2012)
[Konferensbidrag, refereegranskat]

This paper presents an automated algorithm for robustly detecting and segmenting free-lying cell nuclei in bright-field microscope images of Pap smears. This is an essential initial step in the development of an automated screening system for cervical cancer based on malignancy associated change (MAC) analysis. The proposed segmentation algorithm makes use of gray-scale annular closings to identify free-lying nuclei-like objects together with marker-based watershed segmentation to accurately delineate the nuclear boundaries. The algorithm also employs artifact rejection based on size, shape, and granularity to ensure only the nuclei of intermediate squamous epithelial cells are retained. An evaluation of the performance of the algorithm relative to expert manual segmentation of 33 fields-of-view from 11 Pap smear slides is also presented. The results show that the sensitivity and specificity of nucleus detection is 94.71% and 85.30% respectively, and that the accuracy of segmentation, measured using the Dice coefficient, of the detected nuclei is 97.30±1.3%.

Nyckelord: Automated algorithms, Automated screening, Automated segmentation, Brightfield, Cell nucleus, Cervical cancers, Change analysis, Dice coefficient, Epithelial cells, Gray scale, Manual segmentation, Microscope images, Pap smear, Segmentation algorithms, Sensitivity and specificity, Watershed segmentation



Denna post skapades 2013-01-08. Senast ändrad 2016-08-22.
CPL Pubid: 169527

 

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Institutioner (Chalmers)

Institutionen för signaler och system
Institutionen för signaler och system, Digitala bildsystem och bildanalys (1900-2013)

Ämnesområden

Medicinteknik

Chalmers infrastruktur