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In vitro and in vivo degradation of myo-inositol hexakisphosphate by a phytase from Citrobacter braakii

K. Pontoppidan ; V. Glitsoe ; P. Guggenbuhl ; A. P. Quintana ; C. S. Nunes ; D. Pettersson ; Ann-Sofie Sandberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap)
Archives of Animal Nutrition (1745-039X). Vol. 66 (2012), 6, p. 431-444.
[Artikel, refereegranskad vetenskaplig]

Phytases (EC 3.1.3) are widely used in animal feed to increase the availability of phosphorus and decrease the anti nutritive effect of myo-inositol hexakisphosphate (InsP(6)). The aim of this work was to investigate the stereospecific degradation of InsP(6) in vitro and in vivo by a phytase from Citrobacter braakii (C. braakii), and to study gastric survival of the phytase as well as the site of action in the gastrointestinal tract. The in vitro results showed that the C. braakii phytase belongs to the group of 6-phytases (EC 3.1.3.26). However, in approximately one out of 10 instances the phytase initiated hydrolysis at the D-3 (L-1) position, demonstrating that phytase specificity is not unambiguous. Following the main degradation pathway, InsP(6) was degraded by stepwise removal of the phosphate groups on positions 6/1/5. The stereospecificity was found to be similar under in vitro and in vivo conditions. The phytase was found to be stable in the gastric environment and to be active in the stomach and possibly also in the proximal small intestine. While InsP(4) was accumulated under in vitro conditions this was not the case in vivo, where both InsP(5) and InsP(4) were seen to be hydrolysed in the small intestine, possibly as a combined action of the C. braakii phytase and endogenous phosphatases present in the mucosa. The ability of the C. braakii phytase to focus its activity on degrading InsP(6) to InsP(4) is believed to be a favourable complement to the endogenous phosphatases.

Nyckelord: Citrobacter braakii, degradation, inositol phosphates, in vitro, in vivo, phytase, phytate, pigs, degrading enzyme, inositol phosphates, ion chromatography, peniophora-lycii, gastric ph, dephosphorylation, performance, pigs, stereospecificity, isomers



Denna post skapades 2012-12-21. Senast ändrad 2014-11-10.
CPL Pubid: 168569

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)

Ämnesområden

Kemi
Biologiska vetenskaper

Chalmers infrastruktur