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A functioning artificial secretory cell

Lisa Simonsson (Institutionen för teknisk fysik, Biologisk fysik) ; Michael E. Kurczy (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Raphaël Trouillon ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik) ; Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Analytisk kemi)
Scientific Reports (2045-2322). Vol. 2 (2012), p. no. 824.
[Artikel, refereegranskad vetenskaplig]

We present an amperometric study of content release from individual vesicles in an artificial secretory cell designed with the minimal components required to carry out exocytosis. Here, the membranes of the cell and vesicles are substituted for protein-free giant and large unilamellar vesicles respectively. In replacement of the SNARE-complex, the cell model was equipped with an analog composed of complimentary DNA constructs. The DNA constructs hybridize in a zipper-like fashion to bring about docking of the artificial secretory vesicles and following the addition of Ca2+ artificial exocytosis was completed. Exocytotic events recorded from the artificial cell closely approximate exocytosis in live cells. The results together with simulations of vesicular release demonstrate that the molecular flux in this model is attenuated and we suggest that this is the result of restricted diffusion through a semi-stable fusion pore or a partitioning of the signalling molecule out of the fused vesicle membrane.

Nyckelord: chromaffin cells, membrane-fusion, vesicle fusion, exocytotic events, lipid-bilayer, pc12 cells, release, dopamine, amperometry, nanotubes

Denna post skapades 2012-12-14. Senast ändrad 2015-02-26.
CPL Pubid: 167773


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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)
Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)
Institutionen för kemi och molekylärbiologi (GU)


Analytisk kemi
Biokemi och molekylärbiologi

Chalmers infrastruktur

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