CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Immobilization of chondroitin sulfate to lipid membranes and its interactions with ECM proteins

Noomi Altgärde (Institutionen för teknisk fysik, Biologisk fysik) ; Jana Becher ; Stephanie Möller ; Franz E. Weber ; Matthias Schnabelrauch ; Sofia Svedhem (Institutionen för teknisk fysik, Biologisk fysik)
Journal of Colloid and Interface Science (0021-9797). Vol. 390 (2013), p. 528-266.
[Artikel, refereegranskad vetenskaplig]

Glycosaminoglycans (GAGs) in the extracellular matrix (ECM) have multiple functions in tissues including providing support, mediating cell division and differentiation, and taking part in important interactions with proteins, e.g. growth factors. Studying GAG related interactions is inherently difficult and requires suit- able interaction platforms. We show two strategies to covalently couple the GAG chondroitin sulfate (CS) to supported lipid bilayers (SLBs), either by (a) activating carboxy-functionalized phospholipids in the lipid bilayer, followed by the addition of hydrazide-functionalized CS, or by (b) activating naturally occurring carboxyl groups on CS prior to addition to an amino-functionalized SLB. Bilayer formation and subsequent immobilization was followed in real-time using the Quartz Crystal Microbalance with Dissipation monitor- ing, a technique that provides unique information when studying highly hydrated molecular films. The two strategies yielded thin CS films (in the nanometer range) with similar viscoelastic properties. Fluidity of the lipid bilayer was retained when CS was coupled. The application of the CS interaction platform was exemplified for type I collagen and the bone inducing growth factor bone morphogenetic protein-2 (BMP-2). The addition of collagen to immoblized CS resulted in soft layers whereas layers formed by addition of BMP-2 were denser, independent on the immobilization strategy used.

Nyckelord: Supported lipid bilayer Chondroitin sulfate Bone morphogenetic protein-2 (BMP-2) Quartz Crystal Microbalance with Dissipation monitoring (QCM-D)



Denna post skapades 2012-11-14. Senast ändrad 2013-01-02.
CPL Pubid: 166008

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Biologisk fysik
Polymerteknologi
Annan medicinteknik

Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:


Designing biosensor platforms to study glycosaminoglycan interactions


Studies of Glycosaminoglycan Interactions - Surface Immobilization Strategies and Biosensing Applications