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Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids

S. Preus ; Marcus Wilhelmsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Chembiochem (1439-4227). Vol. 13 (2012), 14, p. 1990-2001.
[Artikel, refereegranskad vetenskaplig]

Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for on/off experiments. To use FRET to obtain quantitative distances and three-dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single-molecule levels.

Nyckelord: DNA structures, fluorescence spectroscopy, fluorescent probes, FRET, nucleic acids, resonance energy-transfer, single-molecule fret, alternating-laser, excitation, double-stranded dna, multiparameter fluorescence detection, nano-positioning system, rna-polymerase-ii, probability-distribution, analysis, photoinduced electron-transfer, photon distribution analysis

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Denna post skapades 2012-10-29. Senast ändrad 2015-08-25.
CPL Pubid: 165229


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)


Nanovetenskap och nanoteknik
Biofysikalisk kemi
Biokemi och molekylärbiologi

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