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Effect of protein release rates from tablet formulations on the immune response after sublingual immunization

Författare och institution:
Annika Borde (Institutionen för kemi- och bioteknik, Farmaceutisk teknologi); Annelie Ekman (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi, GU); Jan Holmgren (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi, GU); Anette Larsson (Institutionen för kemi- och bioteknik, Farmaceutisk teknologi)
Publicerad i:
European Journal of Pharmaceutical Sciences, 47 ( 4 ) s. 695-700
ISSN:
0928-0987
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2012
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Dry vaccine formulations for sublingual administration would provide great advantages for public health use, especially in developing countries, since they are easy to administer and might also have improved stability properties. This study investigates the influence of protein release rate from mucoadhesive twolayer tablets on the elicited antibody responses after sublingual immunization. Two fast release tablets, one based on a mixture of lactose and microcrystalline cellulose (MCC) and one protein coated ethylcellulose (EC) tablet, and three hydrophilic matrix tablets with extended release (ER) properties based on HPMC 90 SH 100000 or Carbopol® 974-P NF were tested. The in vitro release profiles of the model protein ovalbumin (OVA) from these tablets were characterized and correlated to the in vivo potential of the tablets to induce an immune response after sublingual immunization in BALB/c mice. It could be concluded that a tablet with fast protein release elicits antibody titres not significantly different from titres obtained with OVA in solution, whereas low immune responses were observed with a slow release of OVA from the ER formulations. Thus, an ER tablet seems not favorable for vaccine delivery to the sublingual mucosa. Thus, we can present a fast releasing tablet formulation with attractive features for sublingual immunization, whereas the use of ER formulations for sublingual vaccination has to be investigated more in detail.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Farmaceutisk vetenskap ->
Galenisk farmaci
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinsk bioteknologi ->
Biomaterialvetenskap
Nyckelord:
Protein delivery Sublingual immunization Fast release Extended release Hydrophilic matrix tablet Ovalbumin
Chalmers styrkeområden:
Livsvetenskaper
Postens nummer:
164573
Ingår i post nr:
Posten skapad:
2012-10-10 15:39
Posten ändrad:
2013-01-17 08:24

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