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Self-assembly formation of multiple DNA-tethered lipid bilayers

Seyed Tabaei (Institutionen för teknisk fysik, Biologisk fysik) ; Peter Jönsson (Institutionen för teknisk fysik, Biologisk fysik) ; Magnus Brändén (Institutionen för teknisk fysik, Biologisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Journal of Structural Biology (1047-8477). Vol. 168 (2009), 1, p. 200-206.
[Artikel, refereegranskad vetenskaplig]

Inspired by natural cell-cell junctions, where membrane-residing proteins control the separation between two or more membranes without interfering with their integrity, we report a new self-assembly route for formation of multiple highly fluid tethered lipid bilayers with the inter-membrane volume geometrically confined by membrane-anchored DNA duplexes. The formation of multiple planar membrane-membrane junctions were accomplished using disk shaped bicelles, composed of a mixture of the long-chained dimyristoyl phosphatidylcholine (DMPC) and the short-chained dihexanoyl PC further stabilized with the positively charged detergent hexadecyl-trimethyl-ammonium bromide (CTAB). Quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy and fluorescence recovery after photobleaching (FRAP) were used to monitor the formation and to characterize the integrity of the self-assembled lipid-DNA architecture. (C) 2009 Elsevier Inc. All rights reserved.



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Denna post skapades 2012-09-11.
CPL Pubid: 163195

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Livsvetenskaper
Nanovetenskap och nanoteknik
Annan teknik
Teknisk fysik

Chalmers infrastruktur