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Five simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequences

R. Henrik Nilsson ; Leho Tedersoo ; Kessy Abarenkov ; Martin Ryberg ; Erik Kristiansson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Martin Hartmann ; Conrad L. Schoch ; Johan A. A. Nylander ; Johannes Bergsten ; Teresita M. Porter ; Ari Jumpponen ; Parag Vaishampayan ; Otso Ovaskainen ; Nils Hallenberg ; Johan Bengtsson ; Karl Martin Eriksson ; Karl-Henrik Larsson ; Ellen Larsson ; Urmas Kõljalg
MycoKeys (1314-4057). Vol. 4 (2012), p. 37-63.
[Artikel, refereegranskad vetenskaplig]

Molecular data form an important research tool in most branches of mycology. A non-trivial proportion of the public fungal DNA sequences are, however, compromised in terms of quality and reliability, contributing noise and bias to sequence-borne inferences such as phylogenetic analysis, diversity assessment, and barcoding. In this paper we discuss various aspects and pitfalls of sequence quality assessment. Based on our observations, we provide a set of guidelines to assist in manual quality management of newly generated, near-full-length (Sanger-derived) fungal ITS sequences and to some extent also sequences of shorter read lengths, other genes or markers, and groups of organisms. The guidelines are intentionally non-technical and do not require substantial bioinformatics skills or significant computational power. Despite their simple nature, we feel they would have caught the vast majority of the severely compromised ITS sequences in the public corpus. Our guidelines are nevertheless not infallible, and common sense and intuition remain important elements in the pursuit of compromised sequence data. The guidelines focus on basic sequence authenticity and reliability of the newly generated sequences, and the user may want to consider additional resources and steps to accomplish the best possible quality control. A discussion on the technical resources for further sequence quality management is therefore provided in the supplementary material.

Nyckelord: ITS, sequence reliability, sequence quality control, fungi, databases, barcoding

Denna post skapades 2012-09-06. Senast ändrad 2015-02-09.
CPL Pubid: 162962


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Institutioner (Chalmers)

Institutionen för biologi och miljövetenskap (GU)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi (GU)


Bioinformatik och systembiologi
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