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Recovery of phenotypes obtained by adaptive evolution through inverse metabolic engineering

inverse metabolic engineering in systems biology

Kuk-Ki Hong (Institutionen för kemi- och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Applied and Environmental Microbiology (0099-2240). Vol. 78 (2012), 21, p. 7579-7586.
[Artikel, refereegranskad vetenskaplig]

In a previous study system level analysis of adaptively evolved yeast mutants showing improved galactose utilization revealed relevant mutations. The governing mutations were suggested to be in the Ras/PKA signaling pathway and ergosterol metabolism. Here site-directed mutants having one of the mutations, RAS2 Lys 77, RAS2 Tyr112 and ERG5 Pro 370 were constructed and evaluated. The mutants were also combined with over-expression of PGM2, earlier proved as a beneficial target for galactose utilization. The constructed strains were analyzed for their gross phenotype, transcriptome and targeted metabolites; and the results were compared to those obtained from reference strains and the evolved strains. The RAS2 Lys 77 mutation resulted in the highest specific galactose uptake rate among all the strains with an increased maximum specific growth rate on galactose. The RAS2 Tyr112 mutation also improved the specific galactose uptake rate and also resulted in many transcriptional changes, including ergosterol metabolism. The ERG5 Pro 370 mutation only showed a small improvement, but when it was combined with PGM2 over-expression, the phenotype was almost the same as that of the evolved mutants. Combination of the RAS2 mutations with PGM2 over-expression also showed a complete recovery of the adaptive phenotype in galactose utilization. Recovery of the gross phenotype by the reconstructed mutants was achieved with much fewer changes in the genome and transcriptome than for the evolved mutants. Our study demonstrates how the identification of specific mutations by systems biology can direct new metabolic engineering strategies for improving galactose utilization by yeast.

Nyckelord: inverse metabolic engineering, evolutionary engineering, systems biology

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Denna post skapades 2012-08-30. Senast ändrad 2014-10-27.
CPL Pubid: 162741


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)


Hållbar utveckling
Bioinformatik och systembiologi
Industriell bioteknik

Chalmers infrastruktur

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Denna publikation ingår i:

Advancing Metabolic Engineering through Combination of Systems Biology and Adaptive Evolution



Denna publikation är ett resultat av följande projekt:

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO) (EC/FP7/247013)