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Chemical images of marine bio-active compounds by surface enhanced Raman spectroscopy and transposed orthogonal partial least squares (T-OPLS)

Aamer Abbas (Institutionen för kemi- och bioteknik) ; M. Josefson ; Göran M. Nylund ; Henrik Pavia ; Katarina Abrahamsson (Institutionen för kemi- och bioteknik)
Analytica Chimica Acta (0003-2670). Vol. 737 (2012), p. 37-44.
[Artikel, refereegranskad vetenskaplig]

Surface enhanced Raman spectroscopy combined with transposed Orthogonal Partial Least Squares (T-OPLS) was shown to produce chemical images of the natural antibacterial surface-active compound 1,1,3,3-tetrabromo-2-heptanone (TBH) on Bonnemaisonia hamifera. The use of gold colloids function-alised with the internal standard 4-mercapto-benzonitrile (MBN) made it possible to create images of the relative concentration of TBH over the surfaces. A gradient of TBH could be mapped over and in the close vicinity of the B. hamifera algal vesicles at the attomol/pixel level. T-OPLS produced a measure of the spectral correlation for each pixel of the hyperspectral images whilst not including spectral variation that was linearly independent of the target spectrum. In this paper we show the possibility to retrieve specific spectral information with a low magnitude in a complex matrix.

Nyckelord: Surface enhanced Raman spectroscopy, Bonnemaisonia hamifera 1,1,3,3-Tetrabromo-2-heptanone, red alga-bonnemaisonia, bacterial-colonization, scattering, pls, chemometrics, fluorescence, feasibility, information, hamifera



Denna post skapades 2012-08-23. Senast ändrad 2013-01-11.
CPL Pubid: 162488

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik (2005-2014)
Institutionen för biologi och miljövetenskap, Tjärnö marinbiologiska laboratorium (GU)
Centrum för marin kemisk ekologi (GU)
Linnécentrum för marin evolutionsbiologi (CEMEB) (GU)

Ämnesområden

Biokemi
Marin ekologi

Chalmers infrastruktur