CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Formation and Diffusivity Characterization of Supported Lipid Bilayers with Complex Lipid Compositions

Lisa Simonsson (Institutionen för teknisk fysik, Biologisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Langmuir (0743-7463). Vol. 28 (2012), 28, p. 10528-10533.
[Artikel, refereegranskad vetenskaplig]

The moving edge of a hydrodynamically manipulated supported lipid bilayer (SLB) can be used to catalyze SLB formation of adsorbed lipid vesicles that do not undergo spontaneous SLB formation upon adsorption on SiO2. By removing the lipid reservoir of an initially formed SLB, we show how a hydrodynamically moved SLB patch composed of POPC can be used to form isolated SLBs with compositions that to at least 95% represent that of the adsorbed lipid vesicles. The concept is used to investigate the diffusivity of lissamine rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (rhodamine-DHPE) in SLBs made from complex lipid compositions, revealing a decrease in diffusivity by a factor of 2 when the cholesterol content was increased from 0% to 50%. We also demonstrate how the concept can be used to induce stationary domains in SLBs containing 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and cholesterol (39:21:40 mol %, respectively). Because the method serves as a means to form SLBs with lipid compositions that hamper SLB formation via spontaneous rupture of adsorbed lipid vesicles, it opens up the possibility for new biophysical investigations of SLBs with more nativelike compositions.

Nyckelord: quartz-crystal microbalance, lateral diffusion, model membranes, planar, membranes, cell-membranes, cholesterol, rafts, mobility, domains, spectroscopy

Denna post skapades 2012-08-21. Senast ändrad 2012-09-19.
CPL Pubid: 162361


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)



Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:

Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics



Denna publikation är ett resultat av följande projekt:

Hydrodynamic concentration of native membrane proteins for on-chip functional studies (VR//2010-5063)