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Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae

Jin Hou (Institutionen för kemi- och bioteknik, Systembiologi) ; Keith Tyo (Institutionen för kemi- och bioteknik, Systembiologi) ; Zihe Liu (Institutionen för kemi- och bioteknik, Systembiologi) ; Dina Petranovic (Institutionen för kemi- och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Fems Yeast Research (1567-1356). Vol. 12 (2012), 5, p. 491-510.
[Artikel, refereegranskad vetenskaplig]

The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels and chemicals, and it is also provides a platform for the production of many heterologous proteins of medical or industrial interest. Therefore, many studies have focused on metabolic engineering S similar to cerevisiae to improve the recombinant protein production, and with the development of systems biology, it is interesting to see how this approach can be applied both to gain further insight into protein production and secretion and to further engineer the cell for improved production of valuable proteins. In this review, the protein post-translational modification such as folding, trafficking, and secretion, steps that are traditionally studied in isolation will here be described in the context of the whole system of protein secretion. Furthermore, examples of engineering secretion pathways, high-throughput screening and systems biology applications of studying protein production and secretion are also given to show how the protein production can be improved by different approaches. The objective of the review is to describe individual biological processes in the context of the larger, complex protein synthesis network.

Nyckelord: protein secretion, systems biology, yeast, genetic engineering, green fluorescent protein, n-linked glycosylation, copii-coated, vesicles, signal recognition particle, allows efficient secretion, er-associated degradation, alpha-factor precursor, endoplasmic-reticulum, heterologous proteins, cell-surface

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Denna post skapades 2012-08-16. Senast ändrad 2014-10-27.
CPL Pubid: 161918


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Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)



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Denna publikation ingår i:

Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae



Denna publikation är ett resultat av följande projekt:

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO) (EC/FP7/247013)