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Synthesis and biodistribution of 211At-labeled, biotinylated, and charge-modified poly-L-lysine: evaluation for use as an effector molecule in pretargeted intraperitoneal tumor therapy.

Sture Lindegren ; Håkan Andersson ; Lars Jacobsson ; Tom Bäck ; Gunnar Skarnemark (Institutionen för material- och ytkemi, Kärnkemi) ; Börje Karlsson
Bioconjugate chemistry (1043-1802). Vol. 13 (2002), 3, p. 502-9.
[Artikel, refereegranskad vetenskaplig]

Poly-L-lysine (7, 21, and 204 kDa) has been evaluated as an effector carrier for use in pretargeted intraperitoneal tumor therapy. For the synthesis, the epsilon-amino groups on the poly-L-lysine were modified in three steps utilizing conjugate biotinylation with biotin amidocaproate N-hydroxysuccinimide ester (BANHS), conjugate radiolabeling with (211)At using the intermediate reagent N-succinimidyl 3-(trimethylstannyl)benzoate (m-MeATE), and charge modification using succinic anhydride, resulting in an increase in the molecular weight of approximately 80% of the final product. The labeling of the m-MeATE reagent and subsequent conjugation of the polymer were highly efficient with overall radiochemical yields in the range of 60-70%. The in vitro avidin binding ability of the modified polymer was almost complete (90-95%), as determined by binding to avidin beads using a convenient filter tube assay. Following intraperitoneal (ip) injection in athymic mice, the 13 kDa polymer product was cleared mainly via the kidneys with fast kinetics (biological half-live T(b) approximately 2 h) and with low whole-body retention. The clearance of the 38 kDa polymer was distributed between kidneys and liver, and the 363 kDa polymer was mainly sequestered by the liver with a T(b) of 8 h. Increased tissue uptake in the thyroid, lungs, stomach, and spleen following the distribution of the large effector molecules (38 and 363 kDa) suggests that degradation of the polymers by the liver may release some of the label as free astatine/astatide.

Nyckelord: Aminocaproic Acids, chemistry, Animals, Astatine, chemistry, Avidin, metabolism, Benzoates, chemistry, Biotin, analogs & derivatives, chemistry, Biotinylation, Drug Carriers, Female, Half-Life, Injections, Intraperitoneal, Liver, metabolism, Mice, Mice, Inbred BALB C, Mice, Nude, Polylysine, chemical synthesis, chemistry, pharmacokinetics, Polymers, pharmacokinetics, Tissue Distribution, Trimethyltin Compounds, chemistry



Denna post skapades 2012-06-11.
CPL Pubid: 158763

 

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Institutioner (Chalmers)

Institutionen för särskilda specialiteter, Avdelningen för radiofysik (1991-2005)
Institutionen för särskilda specialiteter, Avdelningen för onkologi (1991-2005)
Institutionen för material- och ytkemi, Kärnkemi (2002-2004)

Ämnesområden

Fysiologi
Radiofysik

Chalmers infrastruktur