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Non-linear microscopy of smooth muscle cells in artificial extracellular matrices made of cellulose

Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Jan-Olof Dahlberg (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; N. E. Vrana ; C. Lally ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Journal of Biophotonics (1864-063X). Vol. 5 (2012), 5-6, p. 404-414.
[Artikel, refereegranskad vetenskaplig]

Non-linear microscopy has been used to characterize bovine smooth muscle cells and their proliferation, migration, and differentiation in hydrogel cellulose scaffolds, toward the development of fully functional blood vessel implants. The extracellular matrix (ECM) composed of cellulose and endogenous collagen fibers was imaged using Second Harmonic Generation (SHG) microscopy and the cell morphology by Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Images prove that cells adhere on the cellulose scaffold without additional surface modification and that both contractile and proliferating phenotypes are developed. This work shows that non-linear microscopy contributes with unique insights in cell interactions with (artificial) ECM components and has the potential to become an established characterization method in tissue engineering.

Nyckelord: non-linear microscopy, CARS, SHG, tissue engineering, smooth muscle cells, bacterial cellulose, microbial cellulose, collagen, migration, phenotype, scaffolds, substrate, dynamics, adhesions, culture



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Denna post skapades 2012-06-07. Senast ändrad 2017-01-27.
CPL Pubid: 158629

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)
Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)

Ämnesområden

Livsvetenskaper
Kemi

Chalmers infrastruktur