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Mechanistic Insight into the Structure and Dynamics of Entangled and Hydrated lambda-Phage DNA

S. Chakraborty ; Takashi Uematsu (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Christer Svanberg (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Per Jacobsson (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Jan Swenson (Institutionen för teknisk fysik, Kondenserade materiens fysik) ; Michael Zäch (Institutionen för teknisk fysik, Kemisk fysik) ; R. Trehan ; G. Armstrong ; B. Sengupta
Journal of Physical Chemistry A (1089-5639). Vol. 116 (2012), 17, p. 4274-4284.
[Artikel, refereegranskad vetenskaplig]

Intrinsic dynamics of DNA plays a crucial role in DNA protein interactions and has been emphasized as a possible key component for in vivo chromatin organization. We have prepared an entangled DNA microtube above the overlap concentration by exploiting the complementary cohesive ends of lambda-phage DNA, which is confirmed by atomic force microscopy and agarose gel electrophoresis. Photon correlation spectroscopy further confirmed that the entangled solutions are found to exhibit the classical hydrodynamics of a single chain segment on length scales smaller than the hydrodynamic length scale of single lambda-phage DNA molecule. We also observed that in 41.6% (gm water/gm DNA) hydrated state, lambda-phage DNA exhibits a dynamic transition temperature (T-dt) at 187 K and a crossover temperature (T-c) at 246 K. Computational insight reveals that the observed structure and dynamics of entangled lambda-phage DNA are distinctively different from the behavior of the corresponding unentangled DNA with open cohesive ends, which is reminiscent with our experimental observation.

Nyckelord: atomic-force microscopy, light-scattering, anomalous diffusion, aqueous-solutions, circular dna, web server, water, molecules, transition, generation

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Denna post skapades 2012-06-07. Senast ändrad 2012-09-05.
CPL Pubid: 158628


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Institutionen för teknisk fysik, Kondenserade materiens fysik (1900-2015)
Institutionen för teknisk fysik, Kemisk fysik (1900-2015)



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