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A multifunctional pipette

Alar Ainla (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Gavin Jeffries (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; R. Brune ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Aldo Jesorka (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Lab on a Chip (1473-0197). Vol. 12 (2012), 7, p. 1255-1261.
[Artikel, refereegranskad vetenskaplig]

Microfluidics has emerged as a powerful laboratory toolbox for biologists, allowing manipulation and analysis of processes at a cellular and sub-cellular level, through utilization of microfabricated features at size-scales relevant to that of a single cell. In the majority of microfluidic devices, sample processing and analysis occur within closed microchannels, imposing restrictions on sample preparation and use. We present an optimized non-contact open-volume microfluidic tool to overcome these and other restrictions, through the use of a hydrodynamically confined microflow pipette, serving as a multifunctional solution handling and dispensing tool. The geometries of the tool have been optimised for use in optical microscopy, with integrated solution reservoirs to reduce reagent use, contamination risks and cleaning requirements. Device performance was characterised using both epifluorescence and total internal reflection fluorescence (TIRF) microscopy, resulting in similar to 200 ms and similar to 130 ms exchange times at similar to 100 nm and similar to 30 mm distances to the surface respectively.

Nyckelord: push-pull perfusion, microfluidic probe, flow, device, cells



Denna post skapades 2012-04-10. Senast ändrad 2016-04-28.
CPL Pubid: 156490

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Biokemi

Chalmers infrastruktur

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The Multifunctional Pipette. A Microfluidic Technology for the Biosciences