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Microarrays Made Easy: Biofunctionalized Hydrogel Channels for Rapid Protein Microarray Production

V. de Lange ; A. Binkert ; J. Voros ; Marta Bally (Institutionen för teknisk fysik, Biologisk fysik)
ACS Applied Materials & Interfaces (1944-8244). Vol. 3 (2011), 1, p. 50-57.
[Artikel, refereegranskad vetenskaplig]

We present a simple, inexpensive, and sensitive technique for producing multiple copies of a hydrogel-based protein microarray. An agarose block containing 25 biofunctionalized channels is sliced perpendicularly to produce many identical biochips. Each microarray consists of 500 mu m spots, which contain protein-coated microparticles physically trapped in porous SeaPrep agarose. Proteins diffuse readily through SeaPrep agarose while the larger microparticles are immobilized in the hydrogel matrix Without major assay optimization the limit of detection is 12 pM for a sandwich assay detecting human IgG. These highly flexible, multiplexed arrays can produced rapidly without any special instrumentation and are compatible with standard fluorescence-based read-out.

Nyckelord: protein microarray, microparticle-based assay, hydrogel, agarose, microchannel



Denna post skapades 2012-02-22. Senast ändrad 2012-09-19.
CPL Pubid: 155313

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Materialteknik
Medicinsk teknik

Chalmers infrastruktur