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Encapsulated vs. free yeast: A comparative proteomic study

Johan Westman (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Mohammad J. Taherzadeh ; Carl Johan Franzén (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
International PhD course in Industrial Biotechnology for lignocellulose based processes, October 16-21, Göteborg, Sweden (2011)
[Konferensbidrag, poster]

In the search for a replacement for fossil fuels, due to their depletion as well as an increased concern about our environment, 2nd generation bioethanol comes out as one of the most promising alternatives. There are challenges in several steps of lignocellulose processing – especially due to the formation of for yeast inhibitory compounds during pretreatment and hydrolysis. It has previously been shown that encapsulation of the yeast in membranes made of an alginate gel enables the yeast to survive otherwise toxic hydrolysates. The physiological changes arising from encapsulation are however largely unknown, although it has been shown that the macromolecular composition of the yeast changes during prolonged cultivation. In this study we have therefore performed a comparative proteomic study of yeast grown in capsules and in suspension in anaerobic batch cultivations.

Nyckelord: proteomics, bioethanol, biofuel, liquid core encapsulation, 2D-DIGE, nano-LC-MS/MS, Saccharomyces cerevisiae



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Denna post skapades 2012-01-10. Senast ändrad 2014-09-02.
CPL Pubid: 152008

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Energi
Livsvetenskaper
Hållbar utveckling
Biokemi
Funktionsgenomik
Biokemisk och bioteknisk processteknik

Chalmers infrastruktur