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Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids

Marta Bally (Institutionen för teknisk fysik, Biologisk fysik) ; Anders Gunnarsson (Institutionen för teknisk fysik, Biologisk fysik) ; L. Svensson ; Göran Larson ; Vladimir P. Zhdanov (Institutionen för teknisk fysik, Kemisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Physical Review Letters (0031-9007). Vol. 107 (2011), 18,
[Artikel, refereegranskad vetenskaplig]

Glycosphingolipids are involved in the first steps of virus-cell interaction, where they mediate specific recognition of the host cell membrane. We have employed total-internal-reflection fluorescence microscopy to explore the interaction kinetics between individual unlabeled noroviruslike particles, which are attached to a glycosphingolipid-containing lipid bilayer, and fluorescent vesicles containing different types and concentrations of glycosphingolipids. Under association equilibrium, the vesicle-binding rate is found to be kinetically limited, yielding information on the corresponding activation energy. The dissociation kinetics are logarithmic over a wide range of time. The latter is explained by the vesicle-size-related distribution of the dissociation activation energy. The biological, pharmaceutical, and diagnostic relevance of the study is briefly discussed.

Nyckelord: mismatch discrimination, influenza-viruses, infection pathway, norwalk, virus, tracking, bilayers, noroviruses, binding, cells, entry

Denna post skapades 2011-12-02. Senast ändrad 2013-03-15.
CPL Pubid: 149429


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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)
Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (GU)
Institutionen för teknisk fysik, Kemisk fysik (1900-2015)


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