CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Highlights of 20 years of electrochemical measurements of exocytosis at cells and artificial cells

Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Analytisk kemi) ; Andrew G Ewing (Institutionen för kemi- och bioteknik, Analytisk kemi)
Journal of Solid State Electrochemistry (1432-8488). Vol. 15 (2011), 7-8, p. 1437-1450.
[Artikel, refereegranskad vetenskaplig]

Advances in electrochemical methodology over the past 30 years have allowed chemical measurements to be made with decreasing amounts of analyte and at smaller spatial dimensions. This has allowed the investigation of single cells and single vesicles in cells either during release of chemical transmitter or separately. The cellular event called exocytosis can be measured with amperometry or cyclic voltammetry as discovered by Wightman and first published in 1990. In addition, the measurement of vesicle contents with electrochemistry is a new approach we have termed electrochemical cytometry. This involves isolation of intact vesicles, separation of the vesicles, and then lysing followed by coulometric analysis of the electroactive vesicle content. In this review, we will highlight work done by us and by others to discuss measurements of exocytosis at single cells and measurements at artificial cell models for studying the biophysical properties of vesicle membrane dynamics and lipid nanotubes connecting artificial cells using electrochemical methods.

Nyckelord: Vesicle, Exocytosis, Neurotransmitter, Carbon-fiber amperometry, Electrochemical cytometry, Artificial cell, Lipid nanotube, adrenal chromaffin cells, pheochromocytoma pc12 cells, dense core, vesicles, quantal size, plasma-membrane, fusion pore, catecholamine, secretion, transmitter release, patch amperometry, planorbis-corneus, en tk, 1994, v66, p3031, andler de, 1980, v86, p666, en gy, 1995, v15, p7747, ow rh, 1992, v356, p60

Denna post skapades 2011-11-21. Senast ändrad 2015-02-26.
CPL Pubid: 148788


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Analytisk kemi (2006-2014)


Analytisk kemi

Chalmers infrastruktur