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COORDINATION OF MULTIPLE DNA-MOLECULES IN RECA FIBER EVIDENCED BY LINEAR DICHROISM SPECTROSCOPY

Masayuki Takahashi ; Mikael Kubista (Institutionen för fysikalisk kemi) ; Bengt Nordén (Institutionen för fysikalisk kemi)
Biochimie (0300-9084). Vol. 73 (1991), 2-3, p. 219-226.
[Artikel, refereegranskad vetenskaplig]

Polarized light spectroscopy has been used to study the interaction of RecA protein with DNA. Several different DNA complexes have been identified and characterized with respect to stoichiometries, base orientation and nuclease accessibility. By using spectroscopically distinguishable DNAs, we determined the number of DNA molecules co-ordinated by the RecA fiber in each of these complexes, and established their base pairing abilities. Based on these observations, we discuss the molecular mechanism of the RecA-mediated strand exchange reaction.

Nyckelord: reca, recombination, reca-dna complex, linear dichroism, strand exchange, flow-oriented solution, escherichia-coli, electron-microscopy, protein-binding, duplex dna, genetic-recombination, helical filaments, lambda repressor, strand exchange, lexa repressor



Denna post skapades 2011-09-07.
CPL Pubid: 145910

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Biokemi
Molekylärbiologi

Chalmers infrastruktur