CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

EFFECT OF 2.45 GHZ MICROWAVE-RADIATION ON PERMEABILITY OF UNILAMELLAR LIPOSOMES TO 5(6)-CARBOXYFLUORESCEIN - EVIDENCE OF NONTHERMAL LEAKAGE

Elisabeth Saalman (Institutionen för fysikalisk kemi) ; Bengt Nordén (Institutionen för fysikalisk kemi) ; Lars Arvidsson (Institutionen för biokemi och biofysik) ; Yngve Hamnerius (Institutionen för teknisk elektronfysik) ; Per Höjevik (Institutionen för teknisk elektronfysik) ; K. E. Connell ; T. Kurucsev
Biochimica Et Biophysica Acta (0006-3002). Vol. 1064 (1991), 1, p. 124-130.
[Artikel, refereegranskad vetenskaplig]

The influence of 2.45 GHz microwave radiation on the membrane permeability of unilamellar liposomes was studied using the marker 5(6)-carboxyfluorescein trapped in phosphatidylcholine liposomes. The release of the fluorescent marker was followed by spectrofluorimetry after an exposure of 10 minutes to either microwave radiation or to heat alone of the liposome solutions. A significant increase of the permeability of carboxyfluorescein through the membrane was observed for the microwave-exposed samples compared to those exposed to normal heating only. Exposure to 2.45 GHz microwave radiation of liposomes has been previously found to produce increased membrane permeability as compared with heating. However, in contrast to previous studies, the observations reported here were made above the phase transition temperature of the lipid membrane. The experimental setup included monitoring of the temperature during microwave exposure simultaneously at several points in the solution volume using a fiberoptic thermometer. Possible mechanisms to explain the observations are discussed.

Nyckelord: membrane permeability, microwave radiation, liposome, fluorescence, 5(6)-carboxyfluorescein, phase-transition, rabbit erythrocytes, temperature, vesicles, exposure, membrane, efflux



Denna post skapades 2011-09-07.
CPL Pubid: 145897

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)
Institutionen för biokemi och biofysik (1977-1999)
Institutionen för teknisk elektronfysik (1985-1991)

Ämnesområden

Biofysikalisk kemi
Biokemi
Molekylärbiologi

Chalmers infrastruktur